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KMID : 0613820080180050680
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Journal of Life Science
2008 Volume.18 No. 5 p.680 ~ p.685
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Effect of Extracts from Dried Mackerel on Antioxidant Activity and Inhibition of Growth of Human Cancer Cell Lines
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Jang Joo-Ri
Choi Hyung-Ju
Kim Kyung-Kun
Lim Sun-Young
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Abstract
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This study was carried out to determine the inhibitory effects of solvent extracts from dried mackerel on H2O2-induced oxidative stress and growth of cancer cell lines (HT1080 human fibrosarcoma and HT-29 human colon cancer cells). In order to determine the protective effect on H2O2-induced oxidative stress, DCFH-DA (2’,7’-dichlorodihydrofluorescin diacetate) assay was conducted. All fractions including crude extracts of dried mackerel appeared to significantly reduce the levels of intracellular reactive oxygen species (ROS) (p<0.05). Among the fractions, 85% methanol fraction showed a slightly higher protective effect on production of lipid peroxides. Inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts on the growth of HT1080 and HT-29 cancer cells increased in a dose dependent manner (p<0.05) and the inhibitory effect was more significant on the growth of HT1080 cells. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of both cancer cells (p<0.05). These results indicate that the consumption of mackerel may be recommended as a potent functional food for preventing cellular oxidation and cancer.
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KEYWORD
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Dried mackerel, human cancer cell, growth inhibition, antioxidant, reactive oxygen species
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